In this case the mobile phase is a solvent and the stationary phase is made up of particles to which different chemical groups may be bound to allow selective separation of the required analyte.
HPLC – High Performance / High Pressure Liquid Chromatography HPLC is a powerful analytical technique for the separation and analysis of compounds in solution.
HPLC is particularly useful for analysis of large molecules and compounds that are either not very volatile or thermally unstable.
The principles of HPLC are common to other forms of chromatography where separation is based upon chemical interactions with a mobile and stationary phase.
This course will guide you step-by-step through the concepts, techniques and tools necessary to develop validated HPLC methods.
You’ll learn a rapid, systematic approach to HPLC methods development that provides sustainable validation by using statistical process control (SPC) tools.
Rather than developing the HPLC method and then validating it, you’ll be taught a streamlined, iterative process to integrate the method development and validation activities.In addition, by examining case studies in this course, you’ll learn about specific problems and how to approach them.You’ll be learn to carry out routine maintenance to prevent loss of validation, and how to set diagnostics to recognize behavior that requires troubleshooting.Laboratory managers, supervisors, analysts, chemists, biologists, engineers and technicians who are responsible for the continual use of validated high performance liquid chromatographic (HPLC) methods of chemical analysis.Those working in R&D, manufacturing, QA/QC, methods development, process development, product testing, pharmaceuticals, biotechnology, organic chemicals, petroleum, environmental, foods, flavors, fragrances, pesticides, testing services laboratories, and occupational health and safety testing will benefit.The course fee includes a course binder and a continental breakfast each day. Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free.